Kogan G, Pavliak V, Masler L
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Carbohydr Res. 1988 Feb 1;172(2):243-53. doi: 10.1016/s0008-6215(00)90858-9.
A comparative study of three cell-wall mannans, of Candida albicans serotypes A and B and Candida parapsilosis, by means of methylation analysis supports a model of yeast mannans as having an alpha-(1----6)-linked backbone with some units (depending on the origin of the mannan) being substituted at O-2 with oligosaccharides joined by alpha-(1---2) and, to a lesser extent, by alpha-(1----3) glycosidic bonds. Branching points in the side chains of Candida albicans mannans were found in substantial proportions for the first time, and the corresponding branched hexasaccharides were isolated by means of acetolysis and subsequent gel filtration. 13C-N.m.r. spectroscopy of the mannans, as well as a 1H-n.m.r. spectroscopic study of the oligosaccharides obtained on acetolysis of the mannans, led to results that agreed with those of methylation analysis.
通过甲基化分析对白色念珠菌A和B血清型以及近平滑念珠菌的三种细胞壁甘露聚糖进行的比较研究支持了酵母甘露聚糖的模型，即具有α-(1→6)连接的主链，一些单元（取决于甘露聚糖的来源）在O-2位被通过α-(1→2)连接的寡糖取代，在较小程度上被α-(1→3)糖苷键连接的寡糖取代。首次发现白色念珠菌甘露聚糖侧链中有相当比例的分支点，并通过乙酰解和随后的凝胶过滤分离出相应的分支六糖。甘露聚糖的13C核磁共振光谱以及对甘露聚糖乙酰解得到的寡糖的1H核磁共振光谱研究得出的结果与甲基化分析的结果一致。
