Sakagami K
Acta Med Okayama. 1976 Jun;30(3):181-95.
In order to formulate an early diagnostic method for acute rejection after kidney transplantation, macrophage migration inhibition test (MIT) was carried out with lapse of time after inbred rat kidney allotransplantation. The mean survival time of rat kidney allograft was found to be 7.07 +/- 1.34 days. On the other hand, in the group treated with rabbit anti-rat lymphocyte serum (ALS) the mean survival time was lengthened to 14.15 +/- 2.14 days (p less than 0.05). The corresponding antigen used for MIT was prepared with donor kidney by ultrasonication, and its protein concentration at 180 mug/ml was the most optimal as not to elicit non-specific inhibition of macrophages. In the control group, activity of macrophage inhibitory factor (MIF activity) turned positive 3 days after the transplantation, and it became strongly positive by 5 or 7 dyas at the period when rejection crisis appeared frequently. ALS-treated group showed a lower MIF activity than the control group (p less than 0.05) and on 7-12 dyas before rejection crisis appeared frequently, MIF activity became strongly positive. These findings suggest that this MIT is simple and will be proved to be useful in predicting the acute rejection as well as in controlling the immunosuppression.
为了制定一种肾移植后急性排斥反应的早期诊断方法，在近交系大鼠肾同种异体移植后，随着时间的推移进行了巨噬细胞移动抑制试验（MIT）。发现大鼠肾同种异体移植物的平均存活时间为7.07±1.34天。另一方面，在接受兔抗大鼠淋巴细胞血清（ALS）治疗的组中，平均存活时间延长至14.15±2.14天（p<0.05）。用于MIT的相应抗原通过超声处理供体肾制备，其蛋白质浓度为180μg/ml时最为适宜，不会引起巨噬细胞的非特异性抑制。在对照组中，巨噬细胞抑制因子活性（MIF活性）在移植后3天转为阳性，在排斥反应危机频繁出现的5天或7天时变为强阳性。ALS治疗组的MIF活性低于对照组（p<0.05），在排斥反应危机频繁出现前的7至12天，MIF活性变为强阳性。这些发现表明，这种MIT方法简单，将被证明在预测急性排斥反应以及控制免疫抑制方面是有用的。
