重组乳酸乳球菌乳酸亚种在搅拌式发酵罐中的分批培养。II. 混合培养物中的质粒转移。

Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor. II. Plasmid transfer in mixed cultures.

作者信息

el Alami N, Boquien C Y, Corrieu G

机构信息

Institut National de la Recherche Agronomique (INRA), Laboratoire de Génie des Procédés Biotechnologiques Agro-Alimentaires, Thiverval-Grignon, France.

出版信息

Appl Microbiol Biotechnol. 1992 Jun;37(3):364-8. doi: 10.1007/BF00210993.

DOI:10.1007/BF00210993

PMID:1368910

Abstract

The transfer of plasmids was studied in a stirred fermentor in the course of mixed batch cultures combining recombinant strains of Lactococcus lactis subsp. lactis (donor strains) with L. lactis subsp. lactis CNRZ 268M3 (recipient strain). Donor strains contained one or two of the following plasmids (coding for erythromycin or chloramphenicol resistance): pIL205 (self-transmissible), pIL252, pIL253 (non-transmissible but mobilizable by pIL205, respectively small and large copy number) and pE194 (inserted in the chromosome). Only self-transmissible plasmid pIL205 was transferred, with frequencies ranging from 10(-7) to 10(-8) after 12 h of fermentation. These frequencies were 60-400 times lower than in unstirred M17 broth and 100,000 times lower than on agar medium. In the latter case, non-transmissible plasmids pIL252 and pIL253 were mobilized by pIL205 with a frequency of about 10(-5) - 10(-6).

摘要

在搅拌式发酵罐中，结合乳酸乳球菌乳酸亚种的重组菌株（供体菌株）与乳酸乳球菌乳酸亚种CNRZ 268M3（受体菌株）进行分批混合培养的过程中，对质粒转移进行了研究。供体菌株含有以下一种或两种质粒（编码红霉素或氯霉素抗性）：pIL205（自我传递型）、pIL252、pIL253（非传递型，但分别可被pIL205动员，拷贝数少和多）以及插入染色体的pE194。只有自我传递型质粒pIL205发生了转移，发酵12小时后的转移频率范围为10^(-7)至10^(-8)。这些频率比在未搅拌的M17肉汤中低60 - 400倍，比在琼脂培养基上低100,000倍。在后一种情况下，非传递型质粒pIL252和pIL253被pIL205动员，频率约为10^(-5) - 10^(-6)。