Sode K, Tatara M, Takeyama H, Burgess J G, Matsunaga T
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Appl Microbiol Biotechnol. 1992 Jun;37(3):369-73. doi: 10.1007/BF00210994.
Versatility of gene transfer by transconjugation in marine cyanobacteria was demonstrated. In this study, seven different marine cyanobacteria were used as recipient cells. First, transconjugation was carried out using the mobilizable transposon (Tn5) carrying plasmid pSUP1021. Transconjugates were observed in all marine cyanobacteria tested. Second, the broad-host-range vector pKT0230 (IncQ) was tested for transconjugation. pKT230 has been successfully transferred in a marine cyanobacterium Synechococcus sp. NKBG15041C, and replicated as an autonomous replicon without alteration in the restriction enzyme pattern. A maximum transfer efficiency of 5.2 x 10(-4) transconjugants/recipient cell was observed, when mating was performed on agar plates containing low salinity (0.015 M NaCl) medium. This is the first study to demonstrate gene transfer in marine cyanobacteria via transconjugation.
已证明通过转接合在海洋蓝细菌中进行基因转移具有通用性。在本研究中，使用了七种不同的海洋蓝细菌作为受体细胞。首先，使用携带质粒pSUP1021的可移动转座子（Tn5）进行转接合。在所有测试的海洋蓝细菌中都观察到了转接合子。其次，测试了广宿主范围载体pKT0230（IncQ）的转接合情况。pKT230已成功转移到海洋蓝细菌聚球藻属Synechococcus sp. NKBG15041C中，并作为自主复制子进行复制，而限制酶图谱未发生改变。当在含有低盐度（0.015 M NaCl）培养基的琼脂平板上进行交配时，观察到的最大转移效率为5.2×10⁻⁴个转接合子/受体细胞。这是首次证明通过转接合在海洋蓝细菌中进行基因转移的研究。
