Okuda T, Matsuda Y, Sugawara M, Sagisaka S
Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan.
Biosci Biotechnol Biochem. 1992 Dec;56(12):1911-5. doi: 10.1271/bbb.56.1911.
We treated leaves of winter wheat (Triticum aestivum L.) with cold, paraquat, or 3-amino-1,2,4-triazole and compared the responses. We assayed the activities of glucose-6-phosphate dehydrogenase, catalase, dehydroascorbate reductase and ascorbate free radical reductase and levels of hydrogen peroxide, glucose-6-phosphate, fructose-6-phosphate, ascorbate, dehydroascorbate, reduced and oxidized glutathione. With any of the three treatments, contents of cellular peroxides and hexose phosphates were raised. The content of ascorbate was lowered markedly by paraquat treatment, which produces active oxygen species, whereas such a decrease did not occur in other two treatments. When the plants were treated with 3-amino-1,2,4-triazole, which is a specific inhibitor of catalase, the content of oxidized glutathione increased severalfold. The glucose-6-phosphate dehydrogenase activity increased with all three treatments, but it decreased after glyphosate treatment, which does not stimulate the formation of peroxides. The activities of catalase and dehydroascorbate reductase were increased by the treatment of cold and paraquat, while 3-amino-1,2,4-triazole did not affect the dehydroascorbate reductase activity. The activity of ascorbate free radical reductase increased after treatment by paraquat only.
我们用低温、百草枯或3-氨基-1,2,4-三唑处理冬小麦（Triticum aestivum L.）叶片并比较其反应。我们测定了葡萄糖-6-磷酸脱氢酶、过氧化氢酶、脱氢抗坏血酸还原酶和抗坏血酸自由基还原酶的活性以及过氧化氢、葡萄糖-6-磷酸、果糖-6-磷酸、抗坏血酸、脱氢抗坏血酸、还原型和氧化型谷胱甘肽的水平。三种处理中的任何一种都会使细胞过氧化物和己糖磷酸的含量升高。百草枯处理会产生活性氧，显著降低抗坏血酸的含量，而其他两种处理则不会出现这种降低情况。当用作为过氧化氢酶特异性抑制剂的3-氨基-1,2,4-三唑处理植物时，氧化型谷胱甘肽的含量增加了几倍。三种处理均使葡萄糖-6-磷酸脱氢酶活性增加，但草甘膦处理（不刺激过氧化物形成）后该活性降低。低温和百草枯处理会提高过氧化氢酶和脱氢抗坏血酸还原酶的活性，而3-氨基-1,2,4-三唑不影响脱氢抗坏血酸还原酶的活性。仅百草枯处理后抗坏血酸自由基还原酶的活性增加。
