菠菜（Spinacia oleracea）叶绿体中的谷胱甘肽和抗坏血酸。过氧化氢和百草枯的影响。
Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. The effect of hydrogen peroxide and of Paraquat.
作者信息
Law M Y, Charles S A, Halliwell B
出版信息
Biochem J. 1983 Mar 15;210(3):899-903. doi: 10.1042/bj2100899.
Abstract
The stroma of spinach chloroplasts contains ascorbic acid and glutathione at millimolar concentrations. [Reduced glutathione]/[oxidized glutathione] and [ascorbate]/[dehydroascorbate] ratios are high under both light and dark conditions and no evidence for a role of oxidized glutathione or dehydroascorbate in the dark-deactivation of fructose bisphosphatase could be obtained. Addition of H2O2 to chloroplasts in the dark decreases the above ratios, an effect that is reversed on illumination. Addition of Paraquat to illuminated chloroplasts caused a rapid oxidation of reduced glutathione and ascorbate, and apparent loss of dehydroascorbate. Paraquat rapidly inactivated fructose bisphosphatase activity, as assayed under physiological conditions.
摘要
菠菜叶绿体的基质中含有毫摩尔浓度的抗坏血酸和谷胱甘肽。在光照和黑暗条件下，[还原型谷胱甘肽]/[氧化型谷胱甘肽]和[抗坏血酸盐]/[脱氢抗坏血酸盐]的比值都很高，且未获得氧化型谷胱甘肽或脱氢抗坏血酸盐在果糖双磷酸酶暗失活中起作用的证据。在黑暗中向叶绿体中添加过氧化氢会降低上述比值，光照后这种效应会逆转。向光照的叶绿体中添加百草枯会导致还原型谷胱甘肽和抗坏血酸迅速氧化，脱氢抗坏血酸盐明显损失。如在生理条件下测定的那样，百草枯会迅速使果糖双磷酸酶失活。
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本文引用的文献
1
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Plant Physiol. 1981 Jun;67(6):1239-44. doi: 10.1104/pp.67.6.1239.
2
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.
3
Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine.使用谷胱甘肽还原酶和2-乙烯基吡啶测定谷胱甘肽和谷胱甘肽二硫化物。
Anal Biochem. 1980 Jul 15;106(1):207-12. doi: 10.1016/0003-2697(80)90139-6.
4
An improved method for determination of L-ascorbic acid and L-dehydroascorbic acid in blood plasma.一种测定血浆中L-抗坏血酸和L-脱氢抗坏血酸的改进方法。
Clin Chim Acta. 1980 May 9;103(3):259-68. doi: 10.1016/0009-8981(80)90144-8.
5
Formation of hydroxyl radicals from the paraquat radical cation, demonstrated by a highly specific gas chromatographic technique. the role of superoxide radical anion, hydrogen peroxide, and glutathione reductase.通过高度特异性气相色谱技术证明百草枯阳离子自由基形成羟基自由基。超氧阴离子自由基、过氧化氢和谷胱甘肽还原酶的作用。
J Inorg Biochem. 1982 Oct;17(2):95-107. doi: 10.1016/s0162-0134(00)80078-1.
6
Activation of fructose 1,6-bisphosphatase in darkened intact chloroplasts by NADPH.NADPH对黑暗中完整叶绿体果糖1,6-二磷酸酶的激活作用。
Arch Biochem Biophys. 1981 Dec;212(2):644-50. doi: 10.1016/0003-9861(81)90408-2.
7
Molecular properties of chloroplastic thioredoxin f and the photoregulation of the activity of fructose 1,6-bisphosphatase.叶绿体硫氧还蛋白f的分子特性及果糖1,6 -二磷酸酶活性的光调节
Eur J Biochem. 1981 Oct;119(3):497-502. doi: 10.1111/j.1432-1033.1981.tb05635.x.
8
Regulation of fructose-1,6-biphosphatase activity in intact chloroplasts. Studies of the mechanism of inactivation.完整叶绿体中果糖-1,6-二磷酸酶活性的调节。失活机制的研究。
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