Miyamoto K, Ohta H
Department of Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Appl Microbiol Biotechnol. 1992 Nov;38(2):234-8. doi: 10.1007/BF00174474.
We have cloned and sequenced a DNA fragment that encodes the arylmalonate decarboxylase (AM-Dase) gene from Alcaligenes bronchisepticus KU 1201. The AMDase gene consists of an open reading frame of 720 nucleotides, which specifies a 240-amino-acid protein of relative molecular mass (M(r)) 24734. The M(r) deduced from the AMDase gene is in good agreement with that of the AMDase isolated from A. bronchisepticus. No TATA or TTGA sequence was observed within the cloned DNA fragment, but the fragment was expressed in Escherichia coli by the lac promoter of pUC19. The enzyme produced in E. coli has the same M(r) and the same enzyme activity as that purified from A. bronchisepticus. Comparison of the DNA sequence and the deduced amino acid sequence of AMDase with available DNA and amino acid sequence data bases revealed that there are no significant sequence homologies.
我们已克隆并测序了一段DNA片段，该片段编码来自支气管败血波氏杆菌KU 1201的芳基丙二酸脱羧酶（AM - Dase）基因。AM - Dase基因由一个720个核苷酸的开放阅读框组成，它指定了一个相对分子质量（M(r)）为24734的240个氨基酸的蛋白质。从AM - Dase基因推导的M(r)与从支气管败血波氏杆菌分离的AM - Dase的M(r)高度一致。在克隆的DNA片段中未观察到TATA或TTGA序列，但该片段通过pUC19的lac启动子在大肠杆菌中表达。在大肠杆菌中产生的酶与从支气管败血波氏杆菌纯化的酶具有相同的M(r)和相同的酶活性。将AM - Dase的DNA序列和推导的氨基酸序列与现有的DNA和氨基酸序列数据库进行比较，结果表明不存在明显的序列同源性。
