Gähwiler B H
J Neurosci Methods. 1981 Dec;4(4):329-42. doi: 10.1016/0165-0270(81)90003-0.
Cultivation of nervous tissue by means of the roller-tube technique yields thin organotypic cultures. Explants or slices prepared from 1- to 20-day-old rats are embedded in a plasma clot on flying coverslips and cultivated for weeks in roller-tubes. Due to the flattening of the tissue, individual nerve cells are often arranged in monolayer thickness and can, therefore, be viewed with phase-contrast microscopy. This technique is utilized to culture and co-culture nervous tissue derived from various brain regions. The degree of organotypic organization depends on the age of the animals used for culturing. Stable intracellular recordings arae obtained from nerve cells which are impaled under visual control. In view of the accessibility of individual living cells, this approach seems to be particularly well-suited for physiological and pharmacological studies on morphologically identified nerve cells.
通过滚管技术培养神经组织可得到薄的器官型培养物。从1至20日龄大鼠制备的外植体或切片被包埋在飞行盖玻片上的血浆凝块中，并在滚管中培养数周。由于组织的扁平化，单个神经细胞通常以单层厚度排列，因此可以用相差显微镜观察。该技术用于培养和共培养来自不同脑区的神经组织。器官型组织的程度取决于用于培养的动物的年龄。在视觉控制下刺入的神经细胞可获得稳定的细胞内记录。鉴于单个活细胞的可及性，这种方法似乎特别适合于对形态学鉴定的神经细胞进行生理和药理研究。
