Trépanier P, Dugré R, Alain R, Lapierre J
Division of Viral Vaccines, Institut Armand-Frappier, Québec, Canada.
Cytotechnology. 1990 Nov;4(3):261-9. doi: 10.1007/BF00563786.
Obligatory anchorage dependant Vero cells were successfully grown in gelatinous-like macrocarriers made of calcium alginate. Entrapped single cells were immobilized within the polymerized alginate matrix divide to form large spherical clumps of cells. A cell density of 17 x 10(6) cells/ml of alginate with over 95% viability was obtained after 14 days in spinner flasks. When subjected to poliovirus type I infection, spherical masses of Vero cells progressively showed extensive cytopathic effect but remained entrapped in the alginate matrix of the macrocarriers. Virus was released into a cellular debris free-like supernatant and reached a peak titer of 10(8.0) TCID50/ml after 72 hours.
必须依赖贴壁的非洲绿猴肾细胞（Vero细胞）成功地在由海藻酸钙制成的凝胶状大载体中生长。捕获的单个细胞被固定在聚合的海藻酸盐基质中并分裂形成大的球形细胞团。在转瓶中培养14天后，海藻酸盐中的细胞密度达到17×10⁶个细胞/毫升，活力超过95%。当受到I型脊髓灰质炎病毒感染时，Vero细胞的球形团块逐渐出现广泛的细胞病变效应，但仍被困在大载体的海藻酸盐基质中。病毒释放到类似无细胞碎片的上清液中，72小时后达到峰值滴度10⁸.⁰半数组织培养感染剂量（TCID₅₀）/毫升。
