巯基试剂对氯汞苯甲酸使肠道病毒感染性失活
The inactivation of enterovirus infectivity by the sulfhydryl reagent p-chloromercuribenzoate.
作者信息
CHOPPIN P W, PHILIPSON L
出版信息
J Exp Med. 1961 Apr 1;113(4):713-34. doi: 10.1084/jem.113.4.713.
Abstract
The infectivity of several enteroviruses was inactivated by the sulfhydryl reagent p-chloromercuribenzoate (PCMB). The rate of inactivation was dependent on the ionic environment in which the reaction was carried out. Inactivation of infectivity was reversed by the thiol compound, reduced glutathione. Under certain conditions, PCMB prevented the adsorption of some enteroviruses to monolayer cultures of monkey kidney cells. The results suggest that enterovirus sulfhydryl groups are involved in the establishment of infection, and that they play a role in the adsorption of virus to host cells.
摘要
几种肠道病毒的感染性被巯基试剂对氯汞苯甲酸（PCMB）灭活。灭活速率取决于反应进行时的离子环境。感染性的灭活可被硫醇化合物还原型谷胱甘肽逆转。在某些条件下，PCMB可阻止一些肠道病毒吸附到猴肾细胞单层培养物上。结果表明肠道病毒的巯基参与感染的建立，并且它们在病毒吸附到宿主细胞中发挥作用。
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