Kunin C M
J Virol. 1967 Apr;1(2):274-82. doi: 10.1128/JVI.1.2.274-282.1967.
Encephalomyocarditis (EMC) virus was studied for ability to agglutinate erythrocytes of various species. Human, rat, and guinea pig erythrocytes, as well as those from young rabbits, were readily agglutinated. Cells from older rabbits absorbed virus poorly, and showed little agglutination. Uptake of virus by rabbit brain also diminished with age. Various mouse tissues absorbed virus about equally well. Hemagglutination-inhibition studies demonstrated that a number of simple sugars, particularly glucose and galactosamine, interfered with uptake of virus by cells. Dextran sulfates were highly active inhibitors of EMC hemagglutination. Attempts to localize the site of action of the sugars on virus or cell are described. Treatment of virus with periodate or p-chloromercurobenzoate, and acetylation of virus, inhibited hemagglutination, but acetylation of semipurified receptor did not. Clarification of the nature of the virus-cell union will require studies to identify possible specific sugars in the virus capsid and the cell receptor.
对脑心肌炎（EMC）病毒凝集各种物种红细胞的能力进行了研究。人、大鼠和豚鼠的红细胞，以及幼兔的红细胞都很容易被凝集。成年兔的细胞对病毒的吸收较差，且几乎不发生凝集。兔脑对病毒的摄取也随着年龄的增长而减少。各种小鼠组织对病毒的吸收情况大致相同。血凝抑制研究表明，一些单糖，特别是葡萄糖和半乳糖胺，会干扰细胞对病毒的摄取。硫酸葡聚糖是EMC血凝的高效抑制剂。文中描述了确定糖类在病毒或细胞上作用位点的尝试。用过碘酸盐或对氯汞苯甲酸处理病毒以及对病毒进行乙酰化处理均会抑制血凝，但对半纯化受体进行乙酰化处理则不会。要阐明病毒与细胞结合的本质，需要开展研究来鉴定病毒衣壳和细胞受体中可能存在的特定糖类。
