Myxobacteria Bank, Department of Biotechnology, Hoseo University, Asan 336-795, Korea.
J Microbiol Biotechnol. 2013 Mar;23(3):297-303. doi: 10.4014/jmb.1210.10054.
Phylogenetic analysis of the groEL1 and xynB1 gene sequences from Sorangium cellulosum strains isolated in Korea previously revealed the existence of at least 5 subgroups (A-E). In the present study, we used sequence analysis of polymerase chain reaction-amplified biosynthetic genes of strains from the 5 subgroups to indicate correlations between S. cellulosum subgroups and their secondary metabolic gene categories. We detected putative biosynthetic genes for disorazol, epothilone, ambruticin, and soraphen in group A, group C, group D, and group E strains, respectively. With the exception of KYC3204, culture extracts from group A, group B, and group C strains exhibited no noticeable antimicrobial inhibitory activities. By contrast, culture extracts from group D strains inhibited the growth of Candida albicans, whereas culture extracts from group E strains inhibited the growth of C. albicans and Staphylococcus aureus. High performance liquid chromatography analysis of the culture extracts from the strains of each subgroup revealed unique peak patterns. Our findings indicate the existence of at least 5 subgroups of S. cellulosum strains, each of which has the potential to produce a unique set of secondary metabolites.
先前从韩国分离的纤维堆囊菌菌株的 groEL1 和 xynB1 基因序列的系统发育分析显示，至少存在 5 个亚组（A-E）。在本研究中，我们使用来自 5 个亚组的菌株的聚合酶链反应扩增生物合成基因的序列分析来表明纤维堆囊菌亚组与其次级代谢基因类别之间的相关性。我们分别在 A 组、C 组、D 组和 E 组菌株中检测到了推定的 disorazol、epothilone、ambruticin 和 soraphen 的生物合成基因。除了 KYC3204 外，A 组、B 组和 C 组菌株的培养提取物没有表现出明显的抗菌抑制活性。相比之下，D 组菌株的培养提取物抑制了白色念珠菌的生长，而 E 组菌株的培养提取物抑制了白色念珠菌和金黄色葡萄球菌的生长。对每个亚组菌株的培养提取物的高效液相色谱分析显示出独特的峰型。我们的研究结果表明，纤维堆囊菌菌株至少存在 5 个亚组，每个亚组都有可能产生独特的次级代谢产物。
