FARAH F S, KERN M, EISEN H N
J Exp Med. 1960 Dec 1;112(6):1211-26. doi: 10.1084/jem.112.6.1211.
Wheal-and-erythema responses were studied in normal human volunteers and in a single human subject who is sensitive to the 2,4-dinitrophenyl group. In the normal subjects, reactive skin sites were established by intradermal injection of purified rabbit antibody specific for the 2,4-dinitrophenyl group. In both the active and passively sensitized subjects, wheal-and-erythema was elicited by intradermal injection of a 2,4-dinitrophenyl protein, but not by injection of the same conjugate mixed with certain low molecular weight 2,4-dinitrophenyl haptens or with univalent fragments split by papain from anti-2,4-dinitrophenyl antibody. The latter fragments, unlike intact, bivalent, antibody, do not sensitize normal human skin sites. From these and other observations it is concluded that the wheal-and-erythema response in human skin requires mutually multivalent antigen and antibody. This requirement suggests that multimolecular complexes, containing at least 2 antigen and 2 antibody molecules, are essential in the pathogenesis of this allergic response.
在正常人类志愿者和一名对2,4 -二硝基苯基基团敏感的人类受试者中研究了风团和红斑反应。在正常受试者中，通过皮内注射针对2,4 -二硝基苯基基团的纯化兔抗体来建立反应性皮肤部位。在主动致敏和被动致敏的受试者中，皮内注射2,4 -二硝基苯基蛋白可引发风团和红斑，但注射与某些低分子量2,4 -二硝基苯基半抗原混合的相同缀合物或与木瓜蛋白酶从抗2,4 -二硝基苯基抗体裂解得到的单价片段则不会引发。与完整的二价抗体不同，后一种片段不会使正常人类皮肤部位致敏。从这些以及其他观察结果可以得出结论，人类皮肤中的风团和红斑反应需要多价的抗原和抗体相互作用。这一需求表明，至少包含2个抗原分子和2个抗体分子的多分子复合物在这种过敏反应的发病机制中至关重要。
