SALVIN S B, SMITH R F
J Exp Med. 1961 Aug 1;114(2):185-94. doi: 10.1084/jem.114.2.185.
Intradermal injection of a simple hapten (e.g., 1-fluoro-2,4-dinitrobenzene) in water-in-oil emulsion results in contact hypersensitivity to surface application of the homologous hapten and, after appearance of circulating antibody, in Arthus type hypersensitivity to a conjugate of homologous hapten with guinea pig serum. Intradermal administration of this conjugate induces delayed and subsequently Arthus hypersensitivity to the conjugate, but no evidence of a contact reaction to the hapten alone. When a conjugate of hapten plus solubilized guinea pig skin is used as the sensitizing antigen, both contact hypersensitivity to the hapten and delayed and/or Arthus reactions to the conjugate develop. These observations are consistent with the hypothesis that the specificity of contact sensitivity is directed toward some particular protein of the skin which has been modified by combination with hapten.
在油包水乳剂中皮内注射一种简单的半抗原（如1-氟-2,4-二硝基苯），会导致对同源半抗原表面应用产生接触性超敏反应，并且在循环抗体出现后，对同源半抗原与豚鼠血清的结合物产生阿瑟斯型超敏反应。皮内注射这种结合物会诱导对该结合物产生迟发型超敏反应，随后产生阿瑟斯超敏反应，但没有证据表明对单独的半抗原产生接触反应。当使用半抗原加溶解的豚鼠皮肤的结合物作为致敏抗原时，会同时产生对半抗原的接触性超敏反应以及对结合物的迟发型和/或阿瑟斯反应。这些观察结果与以下假设一致，即接触敏感性的特异性针对的是皮肤中某些已通过与半抗原结合而发生改变的特定蛋白质。
