Chang T W, Davis F M, Sun N C, Sun C R, MacGlashan D W, Hamilton R G
Tanox Biosystems, Inc., Houston, Texas 77025.
Biotechnology (N Y). 1990 Feb;8(2):122-6. doi: 10.1038/nbt0290-122.
Monoclonal antibodies (MAbs) specific for surface antigens of lymphocytes are being used to target and deplete tumorous or normal lymphocytes in vivo. Here, we report evidence for the existence of antigenic epitopes on IgE that are accessible on IgE-secreting B cells but not on other cells bearing IgE. Among 42 murine MAbs specific for human IgE, two were shown by fluorescence flow cytometric analyses to bind to IgE-secreting cell lines but not to IgE bound to high-affinity IgE.Fc receptors (Fc epsilon RI) on basophils or low-affinity IgE receptors (Fc epsilon RII) on other cell types. Neither could they induce histamine release from basophils of various donors even under very permissive conditions. These antibodies may be useful for targeting IgE-secreting B cells in patients suffering from IgE-mediated allergies.
针对淋巴细胞表面抗原的单克隆抗体（MAbs）正被用于在体内靶向和清除肿瘤性或正常淋巴细胞。在此，我们报告了IgE上存在抗原表位的证据，这些表位在分泌IgE的B细胞上可及，但在其他携带IgE的细胞上不可及。在42种针对人IgE的鼠单克隆抗体中，通过荧光流式细胞术分析显示，有两种抗体可与分泌IgE的细胞系结合，但不与嗜碱性粒细胞上的高亲和力IgE.Fc受体（FcεRI）或其他细胞类型上的低亲和力IgE受体（FcεRII）结合的IgE结合。即使在非常宽松的条件下，它们也都不能诱导来自不同供体的嗜碱性粒细胞释放组胺。这些抗体可能有助于靶向患有IgE介导的过敏症患者的分泌IgE的B细胞。
