Dille B J, Butzen C C, Birkenmeyer L G
Experimental Biology Research, Abbott Diagnostics Division, North Chicago, Illinois 60064.
J Clin Microbiol. 1993 Mar;31(3):729-31. doi: 10.1128/jcm.31.3.729-731.1993.
Amplification of Chlamydia trachomatis DNA by polymerase chain reaction was compared with amplification by ligase chain reaction (LCR). Both amplification procedures were able to consistently amplify amounts of DNA equivalent to three C. trachomatis elementary bodies. All 15 C. trachomatis serovars were amplified to detectable levels by LCR, and no DNA from 16 organisms potentially found in clinical specimens or from Chlamydia psittaci and Chlamydia pneumoniae was amplified by LCR.
通过聚合酶链反应对沙眼衣原体DNA进行扩增，并与连接酶链反应（LCR）扩增进行比较。两种扩增方法均能始终如一地扩增出相当于三个沙眼衣原体原体的DNA量。所有15种沙眼衣原体血清型均通过LCR扩增至可检测水平，而LCR未扩增出临床标本中可能存在的16种生物体、鹦鹉热衣原体和肺炎衣原体的DNA。
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