FONG J, CHIN D, ELBERG S S
J Exp Med. 1961 Jul 1;114(1):75-87. doi: 10.1084/jem.114.1.75.
Passage of the virulent H37Rv strain of tubercle bacillus in normal or immune systems (normal or immune monocytes suspended in the corresponding serum) resulted in decreased virulence of the bacilli; this was evidenced by the very low mortality rates in mice inoculated intravenously with passaged bacilli. Passaged bacilli when cultivated directly in tween-albumin medium or when grown on glycerol-blood agar plates after recovery from infected mouse tissues proved as virulent as unpassaged bacilli. The decreased virulence of passaged H37Rv was accompanied by loss of ability to bind neutral red. Passaged H37Rv was more sensitive than unpassaged bacilli to inactivation by sodium oleate and by normal monocyte lysate; however, passaged H37Rv was more resistant than unpassaged bacilli to inhibition by streptomycin.
结核杆菌强毒株H37Rv在正常或免疫系统(悬浮于相应血清中的正常或免疫单核细胞)中传代后,其毒力降低;这一点可通过静脉注射传代杆菌的小鼠极低的死亡率得到证明。传代杆菌直接在吐温 - 白蛋白培养基中培养时,或从感染小鼠组织中复苏后在甘油 - 血琼脂平板上生长时,其毒力与未传代杆菌一样。传代H37Rv毒力的降低伴随着结合中性红能力的丧失。传代H37Rv比未传代杆菌对油酸钠和正常单核细胞裂解物的灭活更敏感;然而,传代H37Rv比未传代杆菌对链霉素的抑制更具抗性。