ELBERG S S, FONG J, SCHNEIDER P
J Exp Med. 1956 Oct 1;104(4):455-65. doi: 10.1084/jem.104.4.455.
Studies of the conditions necessary for maintenance of constant cell populations in vitro in the Mackaness type of culture chamber have indicated the importance of preliminary trypsinization of cells and the beneficial effect of 40 per cent rabbit serum in Tyrode's solution. Under these optimal conditions, uninfected suspensions of monocytes exhibited little change in cell numbers over a period of 40 to 72 hours. Infection of monocytes with the virulent H37Rv strain of tubercle bacillus resulted in an early degeneration of a certain proportion of the cells cultivated in the presence of normal rabbit serum. This degeneration was apparent not only for cells of tuberculin-negative animals but also for those derived from tuberculin-positive animals. The serum of animals vaccinated with BCG exerted a favorable effect upon the survival of monocytes infected with virulent tubercle bacilli. Treatment with this serum caused a delay in degeneration of infected normal cells (cells of tuberculin-negative rabbits) and a complete inhibition of degeneration of infected immune cells (cells of rabbits vaccinated with BCG).
对在麦卡尼斯氏培养室中体外维持恒定细胞群体所需条件的研究表明,细胞预先用胰蛋白酶处理很重要,且在泰罗德氏溶液中加入40%兔血清有有益效果。在这些最佳条件下,单核细胞未感染的悬浮液在40至72小时内细胞数量变化很小。用结核杆菌强毒株H37Rv感染单核细胞,会导致在正常兔血清存在下培养的一定比例细胞早期退化。这种退化不仅在结核菌素阴性动物的细胞中明显,在结核菌素阳性动物的细胞中也明显。接种卡介苗动物的血清对感染强毒结核杆菌的单核细胞存活有有利影响。用这种血清处理会导致感染的正常细胞(结核菌素阴性兔的细胞)退化延迟,以及感染的免疫细胞(接种卡介苗兔的细胞)退化完全受到抑制。