Agurell E, Stensman C
Department of Genetic and Cellular Toxicology, Wallenberg Laboratory, Stockholm University, Sweden.
Mutat Res. 1992 Jan-Mar;276(1-2):87-91. doi: 10.1016/0165-1110(92)90057-g.
In a collaborative study on complex mixtures, three complex mixtures and two pure compounds were assayed with the Salmonella microsuspension technique. The two pure compounds were benzo[a]pyrene (BaP) and 1-nitropyrene (1-NP). The three complex mixtures were standard reference materials (SRMs) from the U.S. National Institute of Standards and Technology, SRM 1649, SRM 1650 and SRM 1597. The two samples SRM 1649, an urban dust particulate matter, and SRM 1650, a diesel particulate matter, were sonicated with dichloromethane. Sample SRM 1597 was an extract of a coal tar sample with a complex mixture of polycyclic aromatic hydrocarbons. The microsuspension assay was performed with Salmonella strains TA98 and TA100 according to Kado et al. (1983) with minor modifications (Löfroth et al., 1988). The results showed that the microsuspension technique is a more sensitive assay than the plate incorporation method. Depending on sample, strain and metabolic condition the mutagenic responses were 3-37 times higher in the microsuspension assay than in the conventional plate incorporation assay. The microsuspension method is thus useful for environmental samples which are often available in only small amounts.
在一项关于复杂混合物的合作研究中，采用沙门氏菌微悬浮技术对三种复杂混合物和两种纯化合物进行了检测。这两种纯化合物是苯并[a]芘（BaP）和1-硝基芘（1-NP）。三种复杂混合物是来自美国国家标准与技术研究院的标准参考物质（SRM），即SRM 1649、SRM 1650和SRM 1597。对两个样品，即城市灰尘颗粒物SRM 1649和柴油颗粒物SRM 1650，用二氯甲烷进行了超声处理。样品SRM 1597是一种含有多环芳烃复杂混合物的煤焦油样品提取物。微悬浮试验根据Kado等人（1983年）的方法，对沙门氏菌TA98和TA100菌株进行，仅做了微小修改（Löfroth等人，1988年）。结果表明，微悬浮技术是一种比平板掺入法更灵敏的检测方法。根据样品、菌株和代谢条件的不同，微悬浮试验中的诱变反应比传统平板掺入试验高3至37倍。因此，微悬浮法对于通常仅能获得少量的环境样品很有用。
