Simoneau P, Hu P C
Department of Pediatrics, University of North Carolina, Chapel Hill 27599-7220.
J Bacteriol. 1992 Jan;174(2):627-9. doi: 10.1128/jb.174.2.627-629.1992.
In an effort to make an inventory of the tRNA genes of Mycoplasma pneumoniae, a DNA fragment was found to contain a sequence that can be folded into a hairpin structure very similar to that of the 4.5S RNA of Escherichia coli. Recombinant plasmids carrying this region were able to complement E. coli strains that were deficient in 4.5S RNA. S1 mapping showed that the mature transcript is only 79 nucleotides long.
为了编制肺炎支原体tRNA基因目录,发现一个DNA片段包含一段可折叠成发夹结构的序列,该结构与大肠杆菌4.5S RNA的结构非常相似。携带该区域的重组质粒能够互补缺乏4.5S RNA的大肠杆菌菌株。S1图谱显示成熟转录本仅79个核苷酸长。