GUDNADOTTIR M G
J Exp Med. 1961 Jan 1;113(1):159-76. doi: 10.1084/jem.113.1.159.
Studies on the fate of type 1 polioviruses in two common species of flies were carried out. The amount of virus in carcasses and excreta at different times was determined by the plaque assay method. Flies and their excreta remained infective for 11 days when kept at room temperature or when incubated at 36 degrees C. for 2 hours a day. Flies remained infective for 3 months when kept in hibernation. A relative increase in titer was found to occur between 9 and 18 hours after feeding if the flies were incubated at 36 degrees C. for 5 to 15 hours a day. The peak occurred later, at 40 to 52 hours after feeding if less incubation was used. Titers in excreta were parallel to titers in carcasses. A twofold increase in titer over the initial feeding was observed on 3 occasions with type 1 Mahoney but not with the LSc strain of virus.
对1型脊髓灰质炎病毒在两种常见蝇类中的命运进行了研究。通过蚀斑测定法确定不同时间尸体和排泄物中的病毒量。当置于室温下或每天在36摄氏度孵育2小时时，苍蝇及其排泄物在11天内仍具传染性。当处于冬眠状态时，苍蝇在3个月内仍具传染性。如果每天将苍蝇在36摄氏度孵育5至15小时，喂食后9至18小时之间病毒滴度会相对升高。如果减少孵育时间，峰值会在喂食后40至52小时出现得更晚。排泄物中的滴度与尸体中的滴度平行。在1型马奥尼毒株中，有3次观察到滴度比初始喂食时增加了两倍，但在LSc病毒株中未观察到这种情况。
