Ingolia T D, Craig E A
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2360-4. doi: 10.1073/pnas.79.7.2360.
The primary base sequence of the protein coding regions of the four small heat shock genes of Drosophila melanogaster present at cytological locus 67B has been determined. A single open reading frame large enough to encode a small heat shock protein is found for each gene. The molecular weights of the predicted proteins are in good agreement with experimentally determined values obtained from gel electrophoresis. The predicted amino acid sequences of the four small heat shock genes show striking homologies over approximately 50% of their lengths. This region of extensive homology extends from about amino acid 85 to amino acid 195 out of a total of approximately 200 amino acids. Comparison of the predicted sequence with the known sequences of other proteins revealed a remarkable similarity between this region of homology and the corresponding region of mammalian alpha-crystallin. The possible functional significance of this structural similarity is discussed.
已确定位于果蝇细胞学位点67B处的四个小热休克基因的蛋白质编码区的主要碱基序列。每个基因都发现了一个足够大的单一开放阅读框，可编码一种小热休克蛋白。预测蛋白质的分子量与通过凝胶电泳获得的实验测定值高度一致。四个小热休克基因的预测氨基酸序列在其长度的约50%上显示出惊人的同源性。这种广泛同源性的区域从总共约200个氨基酸中的约第85个氨基酸延伸到第195个氨基酸。将预测序列与其他蛋白质的已知序列进行比较，发现该同源区域与哺乳动物α-晶体蛋白的相应区域之间存在显著相似性。讨论了这种结构相似性可能的功能意义。
