四种小型果蝇热休克蛋白相互关联，且与哺乳动物的α-晶体蛋白相关。
Four small Drosophila heat shock proteins are related to each other and to mammalian alpha-crystallin.
作者信息
Ingolia T D, Craig E A
出版信息
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2360-4. doi: 10.1073/pnas.79.7.2360.
Abstract
The primary base sequence of the protein coding regions of the four small heat shock genes of Drosophila melanogaster present at cytological locus 67B has been determined. A single open reading frame large enough to encode a small heat shock protein is found for each gene. The molecular weights of the predicted proteins are in good agreement with experimentally determined values obtained from gel electrophoresis. The predicted amino acid sequences of the four small heat shock genes show striking homologies over approximately 50% of their lengths. This region of extensive homology extends from about amino acid 85 to amino acid 195 out of a total of approximately 200 amino acids. Comparison of the predicted sequence with the known sequences of other proteins revealed a remarkable similarity between this region of homology and the corresponding region of mammalian alpha-crystallin. The possible functional significance of this structural similarity is discussed.
摘要
已确定位于果蝇细胞学位点67B处的四个小热休克基因的蛋白质编码区的主要碱基序列。每个基因都发现了一个足够大的单一开放阅读框，可编码一种小热休克蛋白。预测蛋白质的分子量与通过凝胶电泳获得的实验测定值高度一致。四个小热休克基因的预测氨基酸序列在其长度的约50%上显示出惊人的同源性。这种广泛同源性的区域从总共约200个氨基酸中的约第85个氨基酸延伸到第195个氨基酸。将预测序列与其他蛋白质的已知序列进行比较，发现该同源区域与哺乳动物α-晶体蛋白的相应区域之间存在显著相似性。讨论了这种结构相似性可能的功能意义。
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Proc Natl Acad Sci U S A. 1982 Apr;79(7):2360-4. doi: 10.1073/pnas.79.7.2360.
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Alterations in translatable ribonucleic acid after heat shock of Saccharomyces cerevisiae.酿酒酵母热休克后可翻译核糖核酸的变化
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Heat shock proteins are methylated in avian and mammalian cells.热休克蛋白在鸟类和哺乳动物细胞中会发生甲基化。
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Translational control of protein synthesis in response to heat shock in D. melanogaster cells.黑腹果蝇细胞中蛋白质合成的翻译控制对热休克的响应。
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Localization of the heat shock-induced proteins in Drosophila melanogaster tissue culture cells.热休克诱导蛋白在黑腹果蝇组织培养细胞中的定位
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A cellular protein that associates with the transforming protein of Rous sarcoma virus is also a heat-shock protein.一种与劳氏肉瘤病毒转化蛋白相关的细胞蛋白也是一种热休克蛋白。
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