Takata R, Izuhara M, Akiyama K
Department of Biotechnology, College of Agriculture, Ehime University, Japan.
Nucleic Acids Res. 1992 Feb 25;20(4):847-50. doi: 10.1093/nar/20.4.847.
The primary transcript of pnp, the gene encoding polynucleotide phosphorylase in Escherichia coli, is processed in the 5' end region by ribonuclease III (RNase III). The unprocessed transcript shows enhanced stability compared with the processed transcript. We report here that, unlike the processed transcript, the unprocessed pnp transcript did not accept endonucleolytic attack at, at least, five cleavage sites. Sequencing analysis of the four cleavage products shows no sequence specific to all these sites, but AU rich stretches were observed at three sites.
编码大肠杆菌多核苷酸磷酸化酶的基因pnp的初级转录本在5'端区域由核糖核酸酶III（RNase III）进行加工。与加工后的转录本相比，未加工的转录本显示出更高的稳定性。我们在此报告，与加工后的转录本不同，未加工的pnp转录本至少在五个切割位点不接受内切核酸酶的攻击。对四种切割产物的测序分析表明，所有这些位点没有特定的序列，但在三个位点观察到富含AU的片段。
