Takata R, Izuhara M, Hori K
Department of Biology, Saga Medical School, Japan.
Nucleic Acids Res. 1989 Sep 25;17(18):7441-51. doi: 10.1093/nar/17.18.7441.
The transcript covering pnp, the gene encoding polynucleotide phosphorylase, is processed by RNaseIII at the 5'-upstream site of the pnp gene. In the RNaseIII-deficient strain, three species of the unprocessed transcript with different lengths could be detected. In this study, the stability of each transcript was analyzed by SI nuclease protection assay. The results show that the half-lives of the unprocessed transcripts are 8 min, whereas the half-life of the processed transcript is 1.5 min. It is also shown that the 5' segment of the unprocessed transcripts is more stable than the middle or the 3' segment.
覆盖多核苷酸磷酸化酶（PNP）编码基因pnp的转录本在pnp基因5'上游位点被RNaseIII加工。在RNaseIII缺陷型菌株中，可以检测到三种不同长度的未加工转录本。在本研究中，通过SI核酸酶保护试验分析了每种转录本的稳定性。结果表明，未加工转录本的半衰期为8分钟，而加工后转录本的半衰期为1.5分钟。还表明，未加工转录本的5'片段比中间或3'片段更稳定。
