斯佩拉比林，具有强大体内活性的新型抗菌抗生素。分类学、发酵、分离及生物活性。

Sperabillins, new antibacterial antibiotics with potent in vivo activity. Taxonomy, fermentation, isolation and biological activity.

作者信息

Katayama N, Nozaki Y, Tsubotani S, Kondo M, Harada S, Ono H

机构信息

Microbiology Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.

出版信息

J Antibiot (Tokyo). 1992 Jan;45(1):10-9. doi: 10.7164/antibiotics.45.10.

DOI:10.7164/antibiotics.45.10

PMID:1372306

Abstract

A Gram-negative bacterium was found to produce new antibacterial antibiotics, sperabillins A, B, C and D, and the producing bacterium was characterized and identified as Pseudomonas fluorescens YK-437. Sperabillins were isolated by column chromatographies using cation-exchange resins, activated carbon and cation-exchange Sephadex, and preparative reverse-phase HPLC. Sperabillins showed antibacterial activity against Gram-negative and Gram-positive bacteria including antibiotic-resistant strains of Pseudomonas aeruginosa and Staphylococcus aureus. Sperabillin A inhibited DNA, RNA, protein, and cell wall biosynthesis in Escherichia coli. Sperabillins showed good protective effects in experimentally infected mice.

摘要

发现一种革兰氏阴性细菌能产生新的抗菌抗生素——斯帕比林A、B、C和D，该产抗生素细菌经鉴定为荧光假单胞菌YK - 437。通过使用阳离子交换树脂、活性炭和阳离子交换葡聚糖凝胶的柱色谱法以及制备型反相高效液相色谱法分离得到斯帕比林。斯帕比林对革兰氏阴性和革兰氏阳性细菌具有抗菌活性，包括铜绿假单胞菌和金黄色葡萄球菌的耐药菌株。斯帕比林A抑制大肠杆菌中的DNA、RNA、蛋白质和细胞壁生物合成。斯帕比林在实验感染小鼠中显示出良好的保护作用。