Katayama N, Tsubotani S, Nozaki Y, Harada S, Ono H
Microbiology Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
J Antibiot (Tokyo). 1990 Mar;43(3):238-46. doi: 10.7164/antibiotics.43.238.
Two new nucleotide antibiotics, fosfadecin and fosfocytocin, have been isolated from the culture filtrates of Pseudomonas viridiflava PK-5 and Pseudomonas fluorescens PK-52, respectively. These antibiotics were purified by column chromatographies using adsorption, gel filtration and ion exchange resins. On the basis of the spectroscopic and degradation studies, the chemical structures of fosfadecin and fosfocytocin were determined. These antibiotics were either enzymatically or chemically hydrolyzed to generate fosfomycin and a new antibiotic, fosfoxacin, which are also produced in the culture filtrates. They showed antibacterial activity against Gram-positive and Gram-negative bacteria. The antibacterial activity of these nucleotide antibiotics was weaker than that of fosfomycin and fosfoxacin.
两种新的核苷酸类抗生素，磷霉素胺和磷胞霉素，分别从黄绿假单胞菌PK-5和荧光假单胞菌PK-52的培养滤液中分离得到。这些抗生素通过使用吸附、凝胶过滤和离子交换树脂的柱色谱法进行纯化。基于光谱和降解研究，确定了磷霉素胺和磷胞霉素的化学结构。这些抗生素通过酶解或化学水解生成磷霉素和一种新的抗生素磷呋新霉素，它们也存在于培养滤液中。它们对革兰氏阳性菌和革兰氏阴性菌均表现出抗菌活性。这些核苷酸类抗生素的抗菌活性比磷霉素和磷呋新霉素弱。
