Schwartz A, Askenase P W, Gershon R K
J Immunol. 1977 Jan;118(1):159-65.
In previous work monoamine depletion due to treatment with reserpine was shown to decrease the elicitabiltiy of DTH responses in mice. In addition, treatment with monoamine oxidase inhibitors prevented the reserpine-induced decrease. These findings led to the suggestion that serotonin-induced increased vascular permeability is necessary to the development of DTH reactions, perhaps by allowing bone marrow-derived macrophage precursor cells, which are obligate components of DTH responses, to migrate through specialized venules into the site of the reaction. We have compared classical drug tachyphlaxis (temporary inhibition of the effects of a drug by prior treatment with agonists) to serotonin in vessels of mouse feet with local inhibition of DTH after serotonin pretreatment of mice. During the tachyphylactic period, DTH responses are depressed. This suggests that serotonin-induced tachyphylaxis of local endothelial receptors can be responsible for DTH inhibition. In contrast, local injection of histamine has no effect on DTH and this drug is a much less potent inducer of tachyphylaxis to serotonin-mediated vasoactive reactions. On the other hand, histamine can inhibit in vitro T cell reactions, which are not affected by serotonin. These data help to further the concept that serotonin plays an important role in the regulation of DTH in mice and that it probably does so by acting on vascular endothelium.
在之前的研究中，已表明用利血平治疗导致的单胺耗竭会降低小鼠迟发型超敏反应（DTH）的激发能力。此外，用单胺氧化酶抑制剂治疗可防止利血平诱导的降低。这些发现提示，血清素诱导的血管通透性增加对于DTH反应的发生是必要的，可能是通过允许骨髓来源的巨噬细胞前体细胞（DTH反应的必需成分）通过特殊小静脉迁移到反应部位。我们将经典药物快速耐受性（通过预先用激动剂治疗对药物作用的暂时抑制）与小鼠足部血管中血清素的快速耐受性进行了比较，并在小鼠经血清素预处理后对DTH进行了局部抑制。在快速耐受期，DTH反应受到抑制。这表明血清素诱导的局部内皮受体快速耐受性可能是DTH抑制的原因。相比之下，局部注射组胺对DTH没有影响，并且该药物对血清素介导的血管活性反应的快速耐受性诱导作用要弱得多。另一方面，组胺可在体外抑制T细胞反应，而血清素对其无影响。这些数据有助于进一步证实血清素在调节小鼠DTH中起重要作用，并且它可能是通过作用于血管内皮来实现的这一概念。
