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使用51Cr标记的单核细胞测量小鼠肺部的细胞免疫反应。

Use of 51Cr-labeled mononuclear cells for measuring the cellular immune response in mouse lungs.

作者信息

Zarkower A, Scheuchenzuber W J, Ferguson F G

出版信息

Infect Immun. 1981 Feb;31(2):712-5. doi: 10.1128/iai.31.2.712-715.1981.

DOI:10.1128/iai.31.2.712-715.1981
PMID:7012018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC351368/
Abstract

Spleen cells labeled with 51Cr were used in sensitized syngeneic mice to measure the degree of mononuclear cell infiltration into antigen-challenged tissues. With this method, increased cellular infiltration was found after footpad challenge of mice sensitized with sheep erythrocyte, Escherichia coli, and BCG antigens. Cellular response also was determined by using this technique in the lungs of mice sensitized with sheep erythrocytes and BCG. This procedure offers the opportunity to measure cellular infiltration, whether due to cellular or humoral influences, in tissues not easily accessible to conventional immunological manipulation.

摘要

用51Cr标记的脾细胞用于致敏的同基因小鼠，以测量单核细胞浸润到抗原攻击组织中的程度。通过这种方法，在用绵羊红细胞、大肠杆菌和卡介苗抗原致敏的小鼠足垫攻击后，发现细胞浸润增加。在用绵羊红细胞和卡介苗致敏的小鼠肺部，也用这种技术测定了细胞反应。该程序提供了一个机会，来测量在常规免疫操作不易触及的组织中，无论是由于细胞还是体液影响引起的细胞浸润。

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引用本文的文献

1
Lung delayed-type hypersensitivity in stressed mice.应激小鼠的肺部迟发型超敏反应。
Can J Comp Med. 1984 Apr;48(2):211-4.

本文引用的文献

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DURATION OF SOME CUTANEOUS HYPERSENSITIVITIES IN MICE.小鼠某些皮肤超敏反应的持续时间
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