Riviere G R, Elliot K S, Adams D F, Simonson L G, Forgas L B, Nilius A M, Lukehart S A
Department of Pediatric Dentistry, Oregon Health Sciences University, Portland.
J Periodontol. 1992 Feb;63(2):131-6. doi: 10.1902/jop.1992.63.2.131.
The purpose of this study was to use monoclonal antibodies to enumerate spirochetes in dental plaque, including the newly recognized pathogen-related oral spirochete (PROS) and specific serovars of Treponema denticola. Plaque was collected from control subjects with no apparent periodontal disease and from sites of moderate to severe chronic periodontitis in patients with inflammatory periodontal disease. Individual monoclonal antibodies were used to determine whether spirochetes were present and then a double-staining protocol was employed to count total spirochetes and specific treponemes in individual microscopic fields. Results indicate that spirochetes are more common at diseased sites and in subgingival plaque than at healthy sites or in supragingival plaque. Together PROS and T. denticola comprised the majority of all spirochetes in all samples and PROS and T. denticola serovars "B" and D were most numerous in plaque from patients with periodontitis. PROS were the majority of all spirochetes in supragingival plaque (76.2% +/- 23.8%) and subgingival plaque (60.9% +/- 19.1%) from periodontitis patients, significantly larger than the percentage of T. denticola serovar "B" (P less than .001 for both supragingival and subgingival plaque) and serovar D (P less than .01 for supragingival and P less than .001 for subgingival plaque). These observations indicate that PROS are the predominant spirochete in plaque from sites of patients with periodontitis, but other analytical approaches are necessary to determine if PROS or T. denticola are pathogenic.
本研究的目的是使用单克隆抗体对牙菌斑中的螺旋体进行计数，包括新发现的与病原体相关的口腔螺旋体（PROS）和齿垢密螺旋体的特定血清型。从无明显牙周疾病的对照受试者以及患有炎症性牙周疾病患者的中度至重度慢性牙周炎部位采集牙菌斑。使用单个单克隆抗体来确定是否存在螺旋体，然后采用双重染色方案对各个显微镜视野中的总螺旋体和特定密螺旋体进行计数。结果表明，螺旋体在患病部位和龈下菌斑中比在健康部位或龈上菌斑中更常见。在所有样本中，PROS和齿垢密螺旋体共同构成了所有螺旋体的大部分，并且在牙周炎患者的牙菌斑中，PROS以及齿垢密螺旋体血清型“B”和“D”数量最多。在牙周炎患者的龈上菌斑（76.2%±23.8%）和龈下菌斑（60.9%±19.1%）中，PROS占所有螺旋体的大部分，显著高于齿垢密螺旋体血清型“B”的百分比（龈上和龈下菌斑均P<0.001）以及血清型“D”的百分比（龈上P<0.01，龈下P<0.001）。这些观察结果表明，PROS是牙周炎患者患病部位牙菌斑中的主要螺旋体，但需要其他分析方法来确定PROS或齿垢密螺旋体是否具有致病性。
