Lal R B, Rudolph D L, Rowe T, Folks T M
Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Immunol. 1992 Mar;12(2):75-83. doi: 10.1007/BF00918136.
Spontaneous lymphocyte proliferation in the absence of exogenous stimulators was examined in asymptomatic HTLV-II-seropositive (n = 12) and seronegative individuals (n = 16). Mean spontaneous lymphocytic proliferation significantly increased on day 8 postculture in HTLV-II-infected individuals (5762 +/- 899 cpm) compared with normal controls (2034 +/- 925 cpm, P less than 0.01). The proliferating cells in infected individuals were predominantly T cells; neither B cells nor monocytes demonstrated any proliferation. Phenotypic analysis of cultured cells from individuals with HTLV-II infection demonstrated differential expression of integrin molecules as defined by anti-CD29 and anti-S6F1 (42.8 +/- 4.2 and 39.6 +/- 5.9%, respectively) on CD8 cells, as compared with day 0 peripheral blood mononuclear cells (PBMC) from infected individuals (19.7 +/- 3.5 and 19.9 +/- 1.9%, respectively) or normal controls (12.9 +/- 3.1 and 11.5 +/- 2.5%, respectively; P less than 0.001 for both comparisons). These CD8+ cells did not express CD16 or CD11b. The culture supernatants derived from the spontaneously proliferating cells had significantly increased levels of sCD8 and sCD25 (765 +/- 180 and 1805 +/- 320 U/ml, respectively) compared with those from normal controls (222 +/- 120 and 305 +/- 90 U/ml, respectively; P less than 0.01). Furthermore, culture supernatants derived from spontaneously proliferating PBMC from HTLV-II-infected individuals had no detectable levels of HTLV antigen and did not stimulate proliferation of PBMC from normal donors. These results suggest that the spontaneous proliferation in HTLV-II asymptomatic carriers is due to expansion of CD8 cells expressing integrin receptors which may serve as costimulatory molecules for their activation.
在无症状的HTLV-II血清阳性个体（n = 12）和血清阴性个体（n = 16）中检测了在无外源性刺激物情况下的自发淋巴细胞增殖情况。与正常对照（2034 +/- 925 cpm）相比，HTLV-II感染个体在培养后第8天的平均自发淋巴细胞增殖显著增加（5762 +/- 899 cpm，P < 0.01）。感染个体中增殖的细胞主要是T细胞；B细胞和单核细胞均未显示任何增殖。对HTLV-II感染个体培养细胞的表型分析表明，与感染个体第0天的外周血单个核细胞（PBMC）（分别为19.7 +/- 3.5%和19.9 +/- 1.9%）或正常对照（分别为12.9 +/- 3.1%和11.5 +/- 2.5%；两项比较P均 < 0.001）相比，由抗CD29和抗S6F1定义的整合素分子在CD8细胞上有差异表达（分别为42.8 +/- 4.2%和39.6 +/- 5.9%）。这些CD8 +细胞不表达CD16或CD11b。与正常对照（分别为222 +/- 120和305 +/- 90 U/ml）相比，自发增殖细胞的培养上清液中sCD8和sCD25水平显著升高（分别为765 +/- 180和1805 +/- 320 U/ml；P < 0.01）。此外，来自HTLV-II感染个体自发增殖PBMC的培养上清液中未检测到HTLV抗原水平，且不刺激正常供体PBMC的增殖。这些结果表明，HTLV-II无症状携带者中的自发增殖是由于表达整合素受体的CD8细胞扩增所致，这些整合素受体可能作为其激活的共刺激分子。
