Volpes R, van den Oord J J, Desmet V J
Department of Pathology, University Hospital Sint-Rafaël, Catholic University of Leuven, Belgium.
Clin Exp Immunol. 1992 Apr;88(1):50-5. doi: 10.1111/j.1365-2249.1992.tb03038.x.
The expression of the selectin receptor LAM-1/Leu 8 was analysed in normal and in inflamed liver tissue, and its expression on mononuclear inflammatory cells was correlated with their topographical distribution in various compartments of the inflamed liver, in order to obtain new insights on possible molecular mechanisms involved in the traffic of mononuclear inflammatory cells throughout the diseased hepatic parenchyma. In normal liver tissue, few scattered mononuclear cells in portal and lobular parenchyma corresponded to both CD4+ and CD8+, as well as to CD45RA+ (2H4+) naive and CD45RO+ (UCHL1+) memory T cells, and were LAM-1/Leu 8+. In acute and chronic inflamed liver biopsies, CD45RO+ (UCHL1+) CD4+ and CD8+ memory T cells largely predominated in both portal and lobular parenchyma. The expression of LAM-1/Leu 8 antigen on these memory T cells varied according to their localization in the liver parenchyma, and it was not correlated with specific aetiological causes. In acute hepatitis, the vast majority of T lymphocytes were LAM-1/Leu 8-. In chronic active hepatitis, memory T cells in portal tracts expressed LAM-1/Leu 8, whereas virtually all intralobular T cells accumulating in areas of periportal and intralobular inflammation were LAM-1/Leu 8-. In chronic persistent hepatitis, the LAM-1/Leu 8+ T cells largely predominated among the numerous mononuclear inflammatory cells within enlarged portal tracts, whereas LAM-1/Leu 8- T cells were restricted to areas of intralobular 'spotty' inflammation. Therefore, two phenotypical populations can be recognized among the memory T cells in inflamed liver tissue, according to their topographical localization: LAM-1/Leu 8+ T cells predominating in portal tracts, and LAM-1/Leu 8- T cells predominating in the lobular parenchyma. These data show that during their migration through the inflamed liver parenchyma, memory T lymphocytes undergo phenotypical changes (LAM-1/Leu 8 shedding) according to their localization in different liver compartments (portal tracts vs. lobular parenchyma), suggesting multiple cellular and molecular mechanisms involved in the regulation of the leucocyte traffic through inflamed liver tissue.
在正常和炎症性肝组织中分析了选择素受体LAM-1/Leu 8的表达，并将其在单核炎性细胞上的表达与其在炎症性肝各区域的拓扑分布相关联，以便深入了解单核炎性细胞在整个患病肝实质中游走可能涉及的分子机制。在正常肝组织中，门脉和小叶实质中少数散在的单核细胞同时表达CD4+和CD8+，以及CD45RA+(2H4+)初始T细胞和CD45RO+(UCHL1+)记忆T细胞，且为LAM-1/Leu 8阳性。在急性和慢性炎症性肝活检组织中，CD45RO+(UCHL1+) CD4+和CD8+记忆T细胞在门脉和小叶实质中均占主导。这些记忆T细胞上LAM-1/Leu 8抗原的表达因其在肝实质中的定位而异，且与特定病因无关。在急性肝炎中，绝大多数T淋巴细胞为LAM-1/Leu 8阴性。在慢性活动性肝炎中，门脉区的记忆T细胞表达LAM-1/Leu 8，而几乎所有聚集在门周和小叶内炎症区域的小叶内T细胞均为LAM-1/Leu 8阴性。在慢性持续性肝炎中，LAM-1/Leu 8阳性T细胞在扩大的门脉区内众多单核炎性细胞中占主导，而LAM-1/Leu 8阴性T细胞局限于小叶内“点状”炎症区域。因此，根据其拓扑定位，在炎症性肝组织中的记忆T细胞中可识别出两个表型群体：在门脉区占主导的LAM-1/Leu 8阳性T细胞和在小叶实质中占主导的LAM-1/Leu 8阴性T细胞。这些数据表明，记忆T淋巴细胞在通过炎症性肝实质迁移过程中，根据其在不同肝区域（门脉区与小叶实质）的定位经历表型变化（LAM-1/Leu 8脱落），提示多种细胞和分子机制参与调节白细胞通过炎症性肝组织的游走。
