Daw M A, Corcoran G D, Falkiner F R, Keane C T
Department of Clinical Microbiology, Trinity College, Dublin, Ireland.
J Hosp Infect. 1992 Mar;20(3):141-51. doi: 10.1016/0195-6701(92)90082-w.
Three methods, O-serotyping, phage typing and susceptibility to bacteriocins, were used to type 357 clinical isolates of Enterobacter cloacae cultured from 219 patients. One hundred and sixty isolates were typed by serology and phage typing. When these two methods were used, primary classification of isolates was based on serology (65.7% typable) and phage typing for further subdivision (94.1% typable). When all the isolates were typed by cloacin susceptibility, 81.5% of them were typable. Maximum discrimination between cultures was achieved when the three methods were used together; no single method was sufficiently discriminatory. There was a close parallel between serotyping and bacteriocin lysis pattern. The latter was easy to perform and the results were achieved within 48 h. By applying this typing system two episodes of cross-infection were identified in a haematology/oncology unit and intensive care unit.
采用三种方法，即O血清分型、噬菌体分型和对细菌素的敏感性，对从219例患者中培养出的357株阴沟肠杆菌临床分离株进行分型。160株分离株通过血清学和噬菌体分型进行分型。使用这两种方法时，分离株的初步分类基于血清学（可分型率为65.7%），然后通过噬菌体分型进行进一步细分（可分型率为94.1%）。当所有分离株通过对阴沟菌素的敏感性进行分型时，其中81.5%可分型。三种方法一起使用时，培养物之间的区分度最大；没有单一方法具有足够的区分能力。血清分型和细菌素裂解模式之间存在密切的平行关系。后者易于操作，结果可在48小时内得出。通过应用这种分型系统，在血液学/肿瘤学病房和重症监护病房发现了两起交叉感染事件。
