Mitchell I C, Turk J L, Mitchell D N
Department of Pathology, Hunterian Institute, Royal College of Surgeons of England, London, U.K.
Lancet. 1992 Apr 25;339(8800):1015-7. doi: 10.1016/0140-6736(92)90536-c.
Because sarcoidosis resembles tuberculosis clinically and histologically, it has been suggested that mycobacteria might have a role in the pathogenesis of the disorder. Mycobacteria have not been found in sarcoid tissues by conventional culture techniques, so we have used a liquid-phase hybridisation method to see whether we could detect mycobacterial rRNA in such tissues. RNA was extracted from five sarcoid and five normal spleens. Extracts were assayed by liquid-phase DNA/RNA hybridisation with a DNA probe specific for the rRNA of the Mycobacterium tuberculosis complex. Hybridisation obtained with the sarcoid spleens, from which mycobacteria were neither seen on microscopy nor cultured with standard methods, was 4.8 times higher than that with normal spleens (p less than 0.001). Our demonstration of mycobacterial nucleic-acid components in sarcoid splenic tissues supports the notion that mycobacteria play a part in the cause of sarcoidosis.
由于结节病在临床和组织学上与结核病相似，有人提出分枝杆菌可能在该疾病的发病机制中起作用。通过传统培养技术未在结节病组织中发现分枝杆菌，因此我们使用液相杂交方法来观察是否能在这类组织中检测到分枝杆菌rRNA。从五个结节病脾脏和五个正常脾脏中提取RNA。提取物通过液相DNA/RNA杂交，使用针对结核分枝杆菌复合群rRNA的DNA探针进行检测。在显微镜下未见到分枝杆菌且用标准方法培养也未培养出分枝杆菌的结节病脾脏中获得的杂交信号，比正常脾脏高4.8倍（p小于0.001）。我们在结节病脾脏组织中证明了分枝杆菌核酸成分，支持了分枝杆菌在结节病病因中起作用的观点。
