Domingo M, Einig C, Eigenbrodt E, Reinacher M
Department of Pathology, School of Veterinary Medicine, University of Giessen, Germany.
J Histochem Cytochem. 1992 May;40(5):665-73. doi: 10.1177/40.5.1374093.
Four monoclonal antibodies (MAb) specific for the L-type isoenzyme of rat pyruvate kinase (L-PK) were produced and characterized. They detect at least two different epitopes of the isoenzyme, as shown in interference binding assay and Western blot analysis after peptide mapping. The MAb were used in immunohistology to demonstrate the L-PK isoenzyme in paraffin-embedded normal rat tissues. L-PK was found only in hepatocytes, kidney proximal tubules, islet cells of pancreas, and epithelial cells of the villi of small intestine. The content of L-PK in hepatocytes was often lower in the periportal areas as compared with the periveneous zone. In kidneys a clearcut difference in L-PK content and distribution existed between male and female rats. Male animals possessed more L-PK in the kidney cortex than females. The L-PK content in the inner cortical zone (straight proximal tubules) was higher than in the outer cortical zone (convoluted proximal tubules) in kidneys of males. In contrast, female rats displayed less L-PK in the inner than in the outer cortical zone of the kidneys. Only some of them exhibited the same amount of the isoenzyme in both parts of the kidney proximal tubules.
制备并鉴定了四种针对大鼠丙酮酸激酶L型同工酶（L-PK）的单克隆抗体（MAb）。如肽图分析后的干扰结合试验和蛋白质免疫印迹分析所示，它们可检测到该同工酶的至少两个不同表位。这些单克隆抗体用于免疫组织学，以显示石蜡包埋的正常大鼠组织中的L-PK同工酶。仅在肝细胞、肾近端小管、胰腺胰岛细胞和小肠绒毛上皮细胞中发现L-PK。与肝小叶周边区相比，肝小叶中央区肝细胞中的L-PK含量通常较低。在肾脏中，雄性和雌性大鼠的L-PK含量及分布存在明显差异。雄性动物肾皮质中的L-PK比雌性动物多。在雄性大鼠的肾脏中，皮质内带（直近端小管）的L-PK含量高于皮质外带（曲近端小管）。相反，雌性大鼠肾脏皮质内带的L-PK比皮质外带少。只有部分雌性大鼠在肾近端小管的两个部位表现出相同量的同工酶。
