使用猫腹水或鼠单克隆抗体在石蜡包埋组织中对猫传染性腹膜炎病毒抗原进行免疫组织学检测。
Immunohistological demonstration of feline infectious peritonitis virus antigen in paraffin-embedded tissues using feline ascites or murine monoclonal antibodies.
作者信息
Tammer R, Evensen O, Lutz H, Reinacher M
机构信息
Inst. Vet.-Univ, Giessen, Germany.
出版信息
Vet Immunol Immunopathol. 1995 Nov;49(1-2):177-82. doi: 10.1016/0165-2427(95)05459-j.
Abstract
Feline infectious peritonitis (FIP) virus antigen was demonstrated after methanol, ethanol or formalin fixation in paraffin-embedded tissues by means of monoclonal and polyclonal antibodies. The monoclonal antibody was induced by immunization with transmissible gastroenteritis virus. Polyclonal antibodies were obtained by purification on protein A-Sepharose of ascites fluid from a cat with FIP. Almost all cats diagnosed as suffering from FIP by postmortem and histological examination exhibited FIP virus (FIPV) antigen in macrophages in granulomas whereas FIPV antigen was only once demonstrable in another location.
摘要
通过单克隆抗体和多克隆抗体，在甲醇、乙醇或福尔马林固定的石蜡包埋组织中证实了猫传染性腹膜炎（FIP）病毒抗原。单克隆抗体是通过用传染性胃肠炎病毒免疫诱导产生的。多克隆抗体是通过对一只患有FIP的猫的腹水进行蛋白A-琼脂糖纯化获得的。几乎所有经尸检和组织学检查诊断为患有FIP的猫，在肉芽肿中的巨噬细胞中都显示出FIP病毒（FIPV）抗原，而FIPV抗原仅在另一个位置被证实过一次。
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