Department of Microbiology, University of Maryland, College Park, Maryland 20742, and The Center of Marine Biotechnology, Baltimore, Maryland 21202.
Appl Environ Microbiol. 1991 Jul;57(7):2094-6. doi: 10.1128/aem.57.7.2094-2096.1991.
The lipopolysaccharides (LPS) of 19 marine bacteria were examined for size heterogeneities by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis in conjunction with an LPS-specific silver staining method. Fifteen marine bacteria had an R-type LPS instead of the ladderlike LPS array characteristic of most bacteria. In addition, three marine bacteria also had a single large LPS molecule. Without constraints (e.g., surface masking), R-type LPS, a more hydrophobic molecule, predominates in Shewanella and Hyphomonas species and in other marine bacteria.
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳结合脂多糖特异性银染方法，检测了 19 种海洋细菌的脂多糖（LPS）的大小异质性。15 种海洋细菌具有 R 型 LPS，而不是大多数细菌所具有的梯状 LPS 排列。此外，三种海洋细菌也有单个大的 LPS 分子。在没有限制（例如，表面掩蔽）的情况下，R 型 LPS 是一种疏水性更强的分子，在希瓦氏菌属和噬氢菌属以及其他海洋细菌中占主导地位。
