Okayama Y, Benyon R C, Rees P H, Lowman M A, Hillier K, Church M K
Clinical Pharmacology, Southampton General Hospital, U.K.
Clin Exp Allergy. 1992 Mar;22(3):401-9. doi: 10.1111/j.1365-2222.1992.tb03102.x.
We have studied an aspect of the functional heterogeneity of human mast cells, namely responsiveness to the inhibitory effects of sodium cromoglycate and nedocromil sodium. The effects of these drugs were examined on the release of histamine and PGD2 from mast cells of human skin, lung, tonsils, adenoids and intestine. A high concentration, 1000 microM, of sodium cromoglycate was required to significantly inhibit histamine release from lung and tonsillar mast cells. Nedocromil sodium, 1000 microM, was more effective than sodium cromoglycate against histamine release from lung, tonsillar and adenoidal cells. Both compounds showed tachyphylaxis in lung and tonsillar mast cells but not in adenoidal and intestinal mast cells. In contrast, in intestinal mast cells, the effect of nedocromil sodium was weaker and more variable than sodium cromoglycate. Skin mast cells differed from mast cells of the other anatomical sites in being unresponsive to sodium cromoglycate and nedocromil sodium. Our results confirm that high concentrations of sodium cromoglycate and nedocromil sodium are required to achieve even modest inhibition of mediator release from human mast cells under in vitro conditions. Notwithstanding this, the results also indicate that differences exist among skin, lung, tonsillar, adenoidal and intestinal mast cells with respect to their sensitivity to sodium cromoglycate and nedocromil sodium, thus extending our knowledge of functional heterogeneity within the human mast cell populations.
我们研究了人类肥大细胞功能异质性的一个方面，即对色甘酸钠和奈多罗米钠抑制作用的反应性。检测了这些药物对人皮肤、肺、扁桃体、腺样体和肠道肥大细胞释放组胺和前列腺素D2的影响。需要1000微摩尔的高浓度色甘酸钠才能显著抑制肺和扁桃体肥大细胞释放组胺。1000微摩尔的奈多罗米钠在抑制肺、扁桃体和腺样体细胞释放组胺方面比色甘酸钠更有效。两种化合物在肺和扁桃体肥大细胞中均表现出快速耐受性，但在腺样体和肠道肥大细胞中则未出现。相比之下，在肠道肥大细胞中，奈多罗米钠的作用比色甘酸钠更弱且更具变异性。皮肤肥大细胞与其他解剖部位的肥大细胞不同，对色甘酸钠和奈多罗米钠无反应。我们的结果证实，在体外条件下，即使要适度抑制人类肥大细胞释放介质，也需要高浓度的色甘酸钠和奈多罗米钠。尽管如此，结果还表明，皮肤、肺、扁桃体、腺样体和肠道肥大细胞对色甘酸钠和奈多罗米钠的敏感性存在差异，从而扩展了我们对人类肥大细胞群体功能异质性的认识。
