Bause-Niedrig I, Jackson M, Schein E, Ludwig H, Pauli G
Institut für Virologie, Freien Universität Berlin, Germany.
Vet Immunol Immunopathol. 1992 Mar;31(3-4):361-9. doi: 10.1016/0165-2427(92)90022-i.
Borna disease virus-specific soluble antigen from persistently infected rat brains was purified to homogeneity using preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The soluble antigen is a complex of three proteins with apparent molecular weights of 35 kDa, 38 kDa and 24 kDa. The 35/38 kDa antigen double band was separated into its two components. The 24 kDa protein has no common epitopes with the 35/38 kDa protein.
使用制备性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，从持续感染的大鼠脑中纯化出博尔纳病病毒特异性可溶性抗原，使其达到均一状态。该可溶性抗原是由三种蛋白质组成的复合物，其表观分子量分别为35 kDa、38 kDa和24 kDa。35/38 kDa抗原双条带被分离成其两个组分。24 kDa蛋白质与35/38 kDa蛋白质没有共同的表位。
