用于检测博尔纳病病毒特异性蛋白抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for detecting antibodies to Borna disease virus-specific proteins.
作者信息
Briese T, Hatalski C G, Kliche S, Park Y S, Lipkin W I
机构信息
Department of Neurology, University of California, Irvine 92717.
出版信息
J Clin Microbiol. 1995 Feb;33(2):348-51. doi: 10.1128/jcm.33.2.348-351.1995.
Abstract
Borna disease virus is a unique neurotropic RNA virus that causes neurologic disease in a wide variety of animal hosts. We established an enzyme-linked immunosorbent assay for the detection of antibodies to Borna disease virus on the basis of the use of three recombinant viral proteins (recp40, recp23, and recp18). This assay system is more sensitive and rapid than the methods currently used for the serologic diagnosis of infection such as Western blotting (immunoblotting), indirect immunofluorescence test, or immunoprecipitation.
摘要
博尔纳病病毒是一种独特的嗜神经RNA病毒，可在多种动物宿主中引发神经系统疾病。我们基于三种重组病毒蛋白（recp40、recp23和recp18）建立了一种用于检测博尔纳病病毒抗体的酶联免疫吸附测定法。该测定系统比目前用于感染血清学诊断的方法（如蛋白质印迹法（免疫印迹法）、间接免疫荧光试验或免疫沉淀法）更灵敏、更快速。
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