Haas B, Becht H, Rott R
J Gen Virol. 1986 Feb;67 ( Pt 2):235-41. doi: 10.1099/0022-1317-67-2-235.
A virus-specific antigen was extracted from brains of rats and from MDCK cells infected with Borna disease (BD) virus and purified to homogeneity by immunoaffinity chromatography and HPLC. The antigen consists of two components which are almost equal in size (38 000 mol. wt.), and it forms aggregates in its native form. The virus specificity of the two antigenic entities was confirmed by immunoblots with convalescent serum and monoclonal antibodies. Immunofluorescent staining with monoclonal antibodies and a hyperimmune serum prepared against the purified antigen showed the intranuclear fluorescence typical for BD virus-infected cells.
从感染博尔纳病（BD）病毒的大鼠脑和MDCK细胞中提取病毒特异性抗原，并通过免疫亲和层析和高效液相色谱法将其纯化至同质。该抗原由两个大小几乎相等（分子量38000）的成分组成，其天然形式会形成聚集体。通过用恢复期血清和单克隆抗体进行免疫印迹，证实了这两种抗原实体的病毒特异性。用单克隆抗体和针对纯化抗原制备的超免疫血清进行免疫荧光染色，显示出BD病毒感染细胞典型的核内荧光。
