Kliche S, Stitz L, Mangalam H, Shi L, Binz T, Niemann H, Briese T, Lipkin W I
Laboratory for Neurovirology, Department of Neurology, University of California, Irvine, 92697, USA.
J Virol. 1996 Nov;70(11):8133-7. doi: 10.1128/JVI.70.11.8133-8137.1996.
Borna disease virus infection is diagnosed by the presence of serum antibodies reactive with the major viral proteins, p40 and p23. Although p40 and p23 are unrelated in amino acid sequence structure, cross-reactive antibodies are described. Protein fragments and synthetic peptides were analyzed to characterize the specificities of antibodies to p23. Epitope mapping revealed eight continuous epitopes accessible on the surface of a predicted structural model for the monomeric and the disulfide-linked dimeric forms of p23. None of these epitopes was reactive with antibodies to p40. Cross-reactivity with monospecific sera and monoclonal antibodies to p40 was found for one discontinuous epitope located at the amino terminus of p23.
博尔纳病病毒感染通过存在与主要病毒蛋白p40和p23发生反应的血清抗体来诊断。尽管p40和p23在氨基酸序列结构上不相关，但仍有交叉反应性抗体的描述。对蛋白质片段和合成肽进行了分析，以表征针对p23的抗体的特异性。表位作图揭示了在p23单体和二硫键连接的二聚体形式的预测结构模型表面上可及的八个连续表位。这些表位均不与针对p40的抗体发生反应。在位于p23氨基末端的一个不连续表位上发现了与针对p40的单特异性血清和单克隆抗体的交叉反应性。
