Suzuki Y, Wang W, Vu T H, Raffin T A
Division of Pulmonary and Critical Care Medicine, Stanford University Medical Center, CA 94305-5236.
Biochem Biophys Res Commun. 1992 May 15;184(3):1339-43. doi: 10.1016/s0006-291x(05)80029-4.
Neutrophil adherence to endothelium is partially mediated by the expression of endothelial leukocyte adhesion molecule-1 (ELAM-1) on endothelial cells activated by agents such as lipopolysaccharide (LPS) and phorbol myristate acetate (PMA). To elucidate molecular mechanisms involved in the induction of ELAM-1 on endothelial cells, we investigated the effect of the NADPH oxidase inhibitor, apocynin (4-hydroxy-3-methoxyacetophenone), on ELAM-1 mRNA expression in human umbilical vein endothelial cells (HUVEC) by Northern blot analysis. Apocynin downregulated both LPS- and PMA-induced ELAM-1 mRNA expression in a dose-dependent manner. Our results suggest NADPH oxidase might play a key role in ELAM-1 mRNA expression in HUVEC.
中性粒细胞与内皮细胞的黏附部分是由内皮细胞上的内皮白细胞黏附分子-1（ELAM-1）的表达介导的，该分子在诸如脂多糖（LPS）和佛波醇肉豆蔻酸酯乙酸酯（PMA）等试剂激活的内皮细胞上表达。为了阐明参与内皮细胞上ELAM-1诱导的分子机制，我们通过Northern印迹分析研究了NADPH氧化酶抑制剂阿朴吗啡（4-羟基-3-甲氧基苯乙酮）对人脐静脉内皮细胞（HUVEC）中ELAM-1 mRNA表达的影响。阿朴吗啡以剂量依赖性方式下调LPS和PMA诱导的ELAM-1 mRNA表达。我们的结果表明，NADPH氧化酶可能在HUVEC中ELAM-1 mRNA表达中起关键作用。
