Kawaguchi Y, Harigai M, Hara M, Suzuki K, Kawakami M, Ishizuka T, Hidaka T, Kitani A, Kawagoe M, Nakamura H
First Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Biochem Biophys Res Commun. 1992 May 15;184(3):1504-10. doi: 10.1016/s0006-291x(05)80053-1.
Summary. To elucidate the mechanisms of the fibrosis in systemic sclerosis (SSc) through the action of a cytokine, interleukin 1 (IL-1), we studied the specific biologic and biochemical features of interleukin 1 receptor (IL-1R) as expressed on the surfaces of fibroblast cells in cultures from 3 SSc patients and 3 normal donors. 125I-IL-1 beta binding assays revealed a high density of IL-1R on the cell surfaces of SSc fibroblasts as compared to those of normal subjects. We also found an enhanced expression of IL-1R messenger RNA (mRNA) in SSc fibroblasts, using Northern blot or slot blot analysis. These findings indicate that the expression of IL-1R on SSc fibroblasts were spontaneously induced at the transcriptional level. It is suggested that SSc fibroblasts are more sensitive to IL-1, and that the signal transduction of IL-1 through IL-1R may be eventually involved in the fibrosis of SSc.
摘要。为了通过细胞因子白细胞介素1（IL-1）的作用来阐明系统性硬化症（SSc）中纤维化的机制，我们研究了来自3名SSc患者和3名正常供体的培养成纤维细胞表面所表达的白细胞介素1受体（IL-1R）的特定生物学和生化特征。125I-IL-1β结合试验显示，与正常受试者相比，SSc成纤维细胞表面的IL-1R密度较高。我们还使用Northern印迹或狭缝印迹分析发现，SSc成纤维细胞中IL-1R信使核糖核酸（mRNA）的表达增强。这些发现表明，SSc成纤维细胞上IL-1R的表达在转录水平上是自发诱导的。提示SSc成纤维细胞对IL-1更敏感，并且IL-1通过IL-1R的信号转导可能最终参与了SSc的纤维化过程。
