Kawaguchi Y, Harigai M, Suzuki K, Hara M, Kobayashi K, Ishizuka T, Matsuki Y, Tanaka N, Nakamura H
First Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Biochem Biophys Res Commun. 1993 Jan 15;190(1):154-61. doi: 10.1006/bbrc.1993.1024.
To determine if SSc fibroblasts are more sensitive to exogenous IL-1 than are normal fibroblasts, we studied the inductions of IL-6, prostaglandin E2 (PGE2), IL-1 beta mRNA production by IL-1-stimulated SSc and normal fibroblasts. The minimal IL-1 beta concentrations for stimulation of IL-6 and PGE2 production by SSc fibroblasts were 10-fold and 100-fold lower, respectively, than those for normal fibroblasts. The minimal IL-1 beta concentration for stimulation of IL-1 beta mRNA expression by SSc fibroblasts was also 10-fold lower than that for normal fibroblasts. These results suggest that the IL-1 signal transduction through IL-1R could be induced excessively in SSc fibroblasts. It is suggested that the IL-1 signal transduction plays an important role in the abnormal cytokine networks observed in the fibrosis of SSc.
为了确定系统性硬化症（SSc）成纤维细胞是否比正常成纤维细胞对外源性白细胞介素-1（IL-1）更敏感，我们研究了IL-1刺激的SSc成纤维细胞和正常成纤维细胞中白细胞介素-6（IL-6）、前列腺素E2（PGE2）、白细胞介素-1β（IL-1β）mRNA的产生情况。刺激SSc成纤维细胞产生IL-6和PGE2的最小IL-1β浓度分别比正常成纤维细胞低10倍和100倍。刺激SSc成纤维细胞表达IL-1βmRNA的最小IL-1β浓度也比正常成纤维细胞低10倍。这些结果表明，通过IL-1受体的IL-1信号转导在SSc成纤维细胞中可能被过度诱导。提示IL-1信号转导在SSc纤维化中观察到的异常细胞因子网络中起重要作用。
