分枝杆菌胞浆内细胞器的精细结构
Fine structures of intracytoplasmic organelles of mycobacteria.
作者信息
KOIKE M, TAKEYA K
出版信息
J Biophys Biochem Cytol. 1961 Mar;9(3):597-608. doi: 10.1083/jcb.9.3.597.
Abstract
The fine structure of the intracytoplasmic organelles of mycobacteria was studied by means of electron microscopy of ultrathin sections. A well-preserved nuclear apparatus was obtained by fixation with OsO(4) in acetate-veronal buffer, containing calcium and tryptone, or in collidine-HCl buffer, followed by uranyl-acetate treatment and embedding in araldite. A low density nuclear region was filled with fine fibrils, 30 A in diameter, in parallel or concentric arrangement. A membranous organelle, tentatively designated as "lamellar structure," consists of unit membranes in lamellar arrangement. The thickness of each lamella in this membranous organelle coincides with that of the three-layered cytoplasmic membrane Moreover, the continuity of this unit membrane with the cytoplasmic membrane was demonstrated.
摘要
通过超薄切片的电子显微镜技术研究了分枝杆菌胞质内细胞器的精细结构。用含有钙和胰蛋白胨的醋酸 - 韦罗那缓冲液或可力丁 - 盐酸缓冲液中的四氧化锇固定，随后进行醋酸铀处理并包埋在环氧树脂中，从而获得保存良好的核装置。低密度核区域充满了直径为30埃的细纤维，呈平行或同心排列。一种暂定为“层状结构”的膜性细胞器由呈层状排列的单位膜组成。该膜性细胞器中每个薄片的厚度与三层细胞质膜的厚度一致。此外，还证明了这种单位膜与细胞质膜的连续性。
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