Peter J B, McKeown K L, Agopian M S
Specialty Laboratories, Inc., Santa Monica, California 90404.
Am J Clin Pathol. 1992 Jun;97(6):858-60. doi: 10.1093/ajcp/97.6.858.
The formulae for immunoglobulin G (IgG) synthesis rate, IgG(loc), IgG index, and IgG extended index in determining increased autochthonous production (intra-blood-brain barrier synthesis) of IgG was assessed in 50 chronic progressive multiple sclerosis patients. The results of all four formulae clearly show elevated intra-blood-brain barrier IgG synthesis in 35 patients, 94% of whom had oligoclonal immunoglobulins detected by the method of isoelectric focusing with immunofixation and silver staining (IEF-IS). The results from the four formulae are all normal in seven patients (none have oligoclonal immunoglobulins by IEF-IS) and are discordant in eight patients (five have oligoclonal immunoglobulins by IEF-IS). Whenever intra-blood-brain barrier synthesis of IgG is suspected, IEF-IS should be used to assay for oligoclonal immunoglobulins because this method is more sensitive than agarose electrophoresis with either Coomassie blue dye or silver stain (94% vs. 74% vs. 54%).
在50例慢性进行性多发性硬化症患者中，评估了免疫球蛋白G（IgG）合成率、IgG（局部）、IgG指数和IgG扩展指数在确定IgG自身合成增加（血脑屏障内合成）方面的公式。所有四个公式的结果均清楚显示，35例患者血脑屏障内IgG合成升高，其中94%的患者通过等电聚焦免疫固定和银染法（IEF-IS）检测到寡克隆免疫球蛋白。七个患者的四个公式结果均正常（通过IEF-IS均未检测到寡克隆免疫球蛋白），八个患者的结果不一致（五个患者通过IEF-IS检测到寡克隆免疫球蛋白）。每当怀疑血脑屏障内有IgG合成时，应使用IEF-IS检测寡克隆免疫球蛋白，因为该方法比考马斯亮蓝染料或银染的琼脂糖电泳更敏感（94%对74%对54%）。
