脊髓灰质炎病毒抗原结构的分子基础：对其在形态发生过程中进化的影响
Molecular basis of antigenic structures of poliovirus: implications for their evolution during morphogenesis.
作者信息
Wiegers K, Dernick R
机构信息
Heinrich-Pette-Institut für Experimentelle Virologie Universität Hamburg, Germany.
出版信息
J Virol. 1992 Jul;66(7):4597-600. doi: 10.1128/JVI.66.7.4597-4600.1992.
Abstract
Neutralizing monoclonal antibodies against poliovirus type 1 were obtained after conventional immunization or combined in vivo-in vitro immunization. Antibody binding sites were determined by sequence analysis of neutralization-resistant mutants. Site 3 variants had several amino acid substitutions in previously unidentified positions for neutralization resistance. Evidence for a linkage of subsites 3a and 3b is presented. Some site 3b antibodies as defined previously precipitated 14S subunits, although with reduced titers.
摘要
通过传统免疫或体内 - 体外联合免疫获得了针对1型脊髓灰质炎病毒的中和单克隆抗体。通过对中和抗性突变体的序列分析确定抗体结合位点。3型位点变体在先前未确定的中和抗性位置有几个氨基酸取代。提供了3a和3b亚位点之间存在联系的证据。一些先前定义的3b型位点抗体沉淀出14S亚基，尽管效价有所降低。
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