Utsuyama M, Hirokawa K, Kurashima C, Fukayama M, Inamatsu T, Suzuki K, Hashimoto W, Sato K
Department of Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.
Mech Ageing Dev. 1992 Mar 15;63(1):57-68. doi: 10.1016/0047-6374(92)90016-7.
Peripheral blood mononuclear cells were obtained from people ranging in age from newborn to 102 years old and analyzed by dual color flow cytometer in terms of number and percentage of various subsets of T cells, B cells and natural killer cells (CD3, 4, 5, 8, 11b, 19, 20, 21, 25, 29, 45RA and 56). Numbers of T cells (CD3+ or CD5+ cells) significantly declined at the 3rd decade as compared with those of younger people, stayed at a relatively constant level between the 3rd and the 7th decade and gradually declined thereafter. In T cell subsets, both CD4 and CD8 positive positive cells decreased with age, but a decrease was more pronounced in the latter, showing an age-related increase of CD4/CD8 ratio. The most interesting finding was a contrasting age-change in two subsets of CD4+ T cells; i.e. a subset of suppressor inducer T cells (CD4+CD45RA+ naive cells) decreased with age, while a subset of helper inducer T cells (CD4+CD29+ memory cells) increased with age. CD20+ B cells also decreased with age in a manner similar to that observed in T cells. Natural killer cells (CD56) showed an increase in numbers with age. The relationship between these changes in various subsets of peripheral blood leukocytes and the age-related decline in immune functions has been discussed.
从年龄范围为新生儿至102岁的人群中获取外周血单个核细胞，并用双色流式细胞仪分析T细胞、B细胞和自然杀伤细胞（CD3、4、5、8、11b、19、20、21、25、29、45RA和56）各亚群的数量和百分比。与年轻人相比，T细胞（CD3+或CD5+细胞）数量在第三个十年显著下降，在第三个和第七个十年之间保持相对稳定水平，此后逐渐下降。在T细胞亚群中，CD4和CD8阳性细胞均随年龄下降，但后者下降更明显，显示出CD4/CD8比值随年龄增加。最有趣的发现是CD4+T细胞的两个亚群呈现相反的年龄变化；即抑制诱导T细胞亚群（CD4+CD45RA+幼稚细胞）随年龄下降，而辅助诱导T细胞亚群（CD4+CD29+记忆细胞）随年龄增加。CD20+B细胞也以与T细胞中观察到的类似方式随年龄下降。自然杀伤细胞（CD56）数量随年龄增加。已讨论外周血白细胞各亚群的这些变化与免疫功能随年龄下降之间的关系。
