特应性疾病患者中CD4+CD45R+和CD4+CD29+辅助性T细胞亚群失衡。
Imbalance of CD4+CD45R+ and CD4+CD29+ T helper cell subsets in patients with atopic diseases.
作者信息
Schauer U, Jung T, Heymanns J, Rieger C H
机构信息
Department of Paediatrics, Philipps University of Marburg, West Germany.
出版信息
Clin Exp Immunol. 1991 Jan;83(1):25-9. doi: 10.1111/j.1365-2249.1991.tb05582.x.
To evaluate the proportion of helper cell subsets we studied 18 children with atopic dermatitis, 30 patients with asthma, 27 healthy age-matched controls aged 1 to 17 years and 11 atopic controls without symptoms related to atopy, aged 9-22 years. Lymphocytes were isolated from heparinized peripheral blood and the proportion of CD4+CD29+ and CD4+CD45R+ cells was determined by double-labelling immunofluorescence. Children with atopic dermatitis yielded a significantly (P less than 0.01) higher proportion of CD4+CD45R+ (median 75%) cells compared with normal controls (median 66.6%), whereas the proportion of CD4+CD29+ cells was significantly (P less than 0.01) lower in patients with atopic dermatitis (median 20.4 versus 29.6%). Interestingly, the percentage of CD4+CD45R+ cells shows an age-dependent decline (r = -0.67, P less than 0.01) in the control group, which is not found in the patient group.
为评估辅助性细胞亚群的比例，我们研究了18例特应性皮炎患儿、30例哮喘患者、27名年龄在1至17岁的年龄匹配的健康对照者以及11名年龄在9至22岁、无特应性相关症状的特应性对照者。从肝素化外周血中分离淋巴细胞，通过双标记免疫荧光法测定CD4+CD29+和CD4+CD45R+细胞的比例。与正常对照者（中位数66.6%）相比，特应性皮炎患儿的CD4+CD45R+细胞比例显著更高（P<0.01，中位数75%），而特应性皮炎患者的CD4+CD29+细胞比例显著更低（P<0.01，中位数分别为20.4%和29.6%）。有趣的是，对照组中CD4+CD45R+细胞百分比呈年龄依赖性下降（r = -0.67，P<0.01），而患者组未发现这种情况。
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