Hayes R J, Tousch D, Jacquemond M, Pereira V C, Buck K W, Tepfer M
Department of Biology, Imperial College of Science, Technology and Medicine, London, U.K.
J Gen Virol. 1992 Jun;73 ( Pt 6):1597-600. doi: 10.1099/0022-1317-73-6-1597.
The 334 nucleotide R satellite RNA was used as a template for purified RNA-dependent RNA polymerase (RdRp) from cucumber mosaic virus-infected tobacco plants. The products of the reaction were dsRNA and positive-strand RNA of the same size as the R satellite RNA. Similar products were obtained when T7 RNA polymerase positive-strand transcripts of a cDNA clone of the satellite RNA, designed to have the same 5' and 3' ends as the satellite RNA, were used as templates. The formation of the positive strands demonstrates complete replication of the satellite RNA. A positive-strand transcript with 65 and 255 additional nucleotides at the 5' and 3' ends of the satellite RNA respectively was also utilized as a template by the RdRp, but only dsRNA was formed. However, no products could be detected when the RdRp was programmed with transcripts corresponding to the negative-strand satellite RNA, either with no additional terminal nucleotides or with 24 and 310 additional nucleotides at the 5' and 3' ends respectively.
将334个核苷酸的R卫星RNA用作从感染黄瓜花叶病毒的烟草植株中纯化的RNA依赖性RNA聚合酶（RdRp）的模板。反应产物为双链RNA和与R卫星RNA大小相同的正链RNA。当具有与卫星RNA相同5'和3'末端的卫星RNA cDNA克隆的T7 RNA聚合酶正链转录本用作模板时，获得了类似的产物。正链的形成表明卫星RNA完全复制。分别在卫星RNA的5'和3'末端带有65和255个额外核苷酸的正链转录本也被RdRp用作模板，但仅形成了双链RNA。然而，当用对应于负链卫星RNA的转录本对RdRp进行编程时，无论是没有额外的末端核苷酸，还是在5'和3'末端分别带有24和310个额外核苷酸，都检测不到产物。
